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        If you use plots from MultiQC in a publication or presentation, please cite:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

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        About MultiQC

        This report was generated using MultiQC, version 1.10.1

        You can see a YouTube video describing how to use MultiQC reports here: https://youtu.be/qPbIlO_KWN0

        For more information about MultiQC, including other videos and extensive documentation, please visit http://multiqc.info

        You can report bugs, suggest improvements and find the source code for MultiQC on GitHub: https://github.com/ewels/MultiQC

        MultiQC is published in Bioinformatics:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

        A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.

        This report has been generated by the nf-core/rnaseq analysis pipeline. For information about how to interpret these results, please see the documentation.

        Report generated on 2024-06-05, 21:22 based on data in: /projects/tomato_genome/fnb/dataprocessing/SRP486761/nfcore-SL5/work/1d/23677d76c725fed5c5bf75a04f3ed4


        General Statistics

        Showing 165/165 rows and 15/19 columns.
        Sample NameM Reads MappeddupInt% Proper PairsError rateM Non-PrimaryM Reads Mapped% Mapped% Proper PairsM Total seqs% AlignedM Aligned% BP Trimmed% Dups% GCM Seqs
        SRR27782291
        98.5
        0.00%
        73.5%
        0.20%
        21.8
        76.6
        100.0%
        100.0%
        76.6
        91.0%
        36.2
        SRR27782291_1
        1.6%
        77.8%
        42%
        39.8
        SRR27782291_2
        1.5%
        79.1%
        43%
        39.8
        SRR27782292
        96.5
        0.00%
        83.8%
        0.21%
        12.7
        83.8
        100.0%
        100.0%
        83.8
        93.5%
        40.4
        SRR27782292_1
        0.9%
        78.0%
        41%
        43.2
        SRR27782292_2
        0.9%
        79.0%
        42%
        43.2
        SRR27782293
        44.2
        0.00%
        82.5%
        0.24%
        6.3
        37.9
        100.0%
        100.0%
        37.9
        92.4%
        18.2
        SRR27782293_1
        0.8%
        71.0%
        42%
        19.7
        SRR27782293_2
        0.9%
        72.2%
        43%
        19.7
        SRR27782294
        49.9
        0.00%
        73.2%
        0.20%
        11.3
        38.6
        100.0%
        100.0%
        38.6
        90.2%
        18.3
        SRR27782294_1
        1.3%
        69.9%
        42%
        20.3
        SRR27782294_2
        1.3%
        72.0%
        42%
        20.3
        SRR27782295
        85.1
        0.00%
        72.9%
        0.19%
        19.3
        65.8
        100.0%
        100.0%
        65.8
        91.4%
        31.0
        SRR27782295_1
        1.1%
        75.9%
        42%
        33.9
        SRR27782295_2
        1.1%
        77.3%
        43%
        33.9
        SRR27782296
        93.4
        0.00%
        89.8%
        0.30%
        7.2
        86.2
        100.0%
        100.0%
        86.2
        94.8%
        42.0
        SRR27782296_1
        0.7%
        70.7%
        41%
        44.3
        SRR27782296_2
        0.8%
        71.1%
        41%
        44.3
        SRR27782297
        94.2
        0.00%
        82.7%
        0.27%
        13.2
        81.0
        100.0%
        100.0%
        81.0
        93.6%
        39.0
        SRR27782297_1
        0.8%
        74.4%
        40%
        41.6
        SRR27782297_2
        0.8%
        74.7%
        41%
        41.6
        SRR27782298
        75.0
        0.00%
        82.3%
        0.29%
        10.8
        64.2
        100.0%
        100.0%
        64.2
        87.9%
        30.9
        SRR27782298_1
        1.2%
        73.5%
        39%
        35.1
        SRR27782298_2
        1.0%
        73.2%
        41%
        35.1
        SRR27782299
        80.4
        0.00%
        82.3%
        0.28%
        11.5
        68.9
        100.0%
        100.0%
        68.9
        90.2%
        33.1
        SRR27782299_1
        1.0%
        76.1%
        39%
        36.7
        SRR27782299_2
        0.9%
        76.1%
        41%
        36.7
        SRR27782300
        84.7
        0.00%
        82.9%
        0.26%
        11.7
        73.1
        100.0%
        100.0%
        73.1
        92.6%
        35.2
        SRR27782300_1
        1.1%
        75.6%
        39%
        38.0
        SRR27782300_2
        1.0%
        76.1%
        41%
        38.0
        SRR27782301
        68.9
        0.00%
        87.1%
        0.27%
        7.0
        62.0
        100.0%
        100.0%
        62.0
        93.3%
        30.0
        SRR27782301_1
        1.1%
        73.2%
        40%
        32.2
        SRR27782301_2
        1.0%
        73.7%
        41%
        32.2
        SRR27782302
        82.9
        0.00%
        86.0%
        0.29%
        9.2
        73.7
        100.0%
        100.0%
        73.7
        94.1%
        35.7
        SRR27782302_1
        0.9%
        72.1%
        40%
        37.9
        SRR27782302_2
        0.9%
        72.8%
        41%
        37.9
        SRR27782303
        90.4
        0.00%
        80.7%
        0.25%
        14.4
        76.1
        100.0%
        100.0%
        76.1
        93.2%
        36.5
        SRR27782303_1
        1.0%
        74.8%
        40%
        39.2
        SRR27782303_2
        0.9%
        75.4%
        41%
        39.2
        SRR27782304
        82.3
        0.00%
        84.1%
        0.28%
        10.5
        71.8
        100.0%
        100.0%
        71.8
        94.3%
        34.6
        SRR27782304_1
        0.8%
        72.9%
        41%
        36.7
        SRR27782304_2
        0.8%
        73.2%
        41%
        36.7
        SRR27782305
        108.6
        0.00%
        74.4%
        0.26%
        22.9
        85.6
        100.0%
        100.0%
        85.6
        91.2%
        40.4
        SRR27782305_1
        1.0%
        77.7%
        40%
        44.3
        SRR27782305_2
        0.9%
        77.9%
        41%
        44.3
        SRR27782306
        79.0
        0.00%
        81.7%
        0.28%
        11.8
        67.2
        100.0%
        100.0%
        67.2
        93.4%
        32.3
        SRR27782306_1
        1.0%
        72.1%
        40%
        34.6
        SRR27782306_2
        0.9%
        72.3%
        41%
        34.6
        SRR27782307
        93.4
        0.00%
        83.6%
        0.31%
        12.4
        81.0
        100.0%
        100.0%
        81.0
        94.2%
        39.0
        SRR27782307_1
        0.8%
        71.0%
        41%
        41.5
        SRR27782307_2
        0.8%
        71.2%
        41%
        41.5
        SRR27782308
        128.2
        0.00%
        52.1%
        0.29%
        53.2
        75.0
        100.0%
        100.0%
        75.0
        87.2%
        33.4
        SRR27782308_1
        0.9%
        73.3%
        42%
        38.3
        SRR27782308_2
        0.9%
        73.5%
        42%
        38.3
        SRR27782309
        88.2
        0.00%
        87.8%
        0.29%
        8.5
        79.7
        100.0%
        100.0%
        79.7
        94.8%
        38.7
        SRR27782309_1
        0.8%
        70.7%
        41%
        40.8
        SRR27782309_2
        0.9%
        71.2%
        41%
        40.8
        SRR27782310
        79.2
        0.00%
        87.1%
        0.28%
        8.1
        71.0
        100.0%
        100.0%
        71.0
        94.8%
        34.5
        SRR27782310_1
        0.9%
        71.5%
        40%
        36.4
        SRR27782310_2
        0.9%
        72.4%
        41%
        36.4
        SRR27782311
        67.3
        0.00%
        84.7%
        0.29%
        8.3
        59.1
        100.0%
        100.0%
        59.1
        93.2%
        28.5
        SRR27782311_1
        0.9%
        72.5%
        40%
        30.6
        SRR27782311_2
        0.9%
        73.5%
        42%
        30.6
        SRR27782312
        58.4
        0.00%
        89.5%
        0.29%
        4.7
        53.7
        100.0%
        100.0%
        53.7
        92.7%
        26.1
        SRR27782312_1
        1.1%
        75.8%
        40%
        28.2
        SRR27782312_2
        1.0%
        75.7%
        41%
        28.2
        SRR27782313
        71.8
        0.00%
        84.5%
        0.26%
        8.9
        62.9
        100.0%
        100.0%
        62.9
        93.9%
        30.4
        SRR27782313_1
        0.9%
        71.0%
        41%
        32.3
        SRR27782313_2
        0.9%
        72.6%
        42%
        32.3
        SRR27782314
        112.9
        0.00%
        61.7%
        0.30%
        37.0
        75.9
        100.0%
        100.0%
        75.9
        87.2%
        34.8
        SRR27782314_1
        0.7%
        72.1%
        41%
        40.0
        SRR27782314_2
        0.8%
        72.3%
        42%
        40.0
        SRR27782315
        81.8
        0.00%
        87.3%
        0.29%
        8.2
        73.6
        100.0%
        100.0%
        73.6
        94.2%
        35.7
        SRR27782315_1
        0.8%
        71.6%
        40%
        37.9
        SRR27782315_2
        0.8%
        71.9%
        41%
        37.9
        SRR27782316
        115.4
        0.00%
        86.1%
        0.27%
        12.7
        102.7
        100.0%
        100.0%
        102.7
        94.0%
        49.7
        SRR27782316_1
        0.8%
        76.4%
        40%
        52.9
        SRR27782316_2
        0.8%
        76.4%
        41%
        52.9
        SRR27782317
        83.5
        0.00%
        88.4%
        0.27%
        7.5
        76.0
        100.0%
        100.0%
        76.0
        94.7%
        36.9
        SRR27782317_1
        0.8%
        70.4%
        41%
        39.0
        SRR27782317_2
        0.8%
        71.3%
        41%
        39.0
        SRR27782318
        90.8
        0.00%
        89.0%
        0.25%
        7.7
        83.0
        100.0%
        100.0%
        83.0
        95.0%
        40.4
        SRR27782318_1
        0.9%
        71.7%
        41%
        42.5
        SRR27782318_2
        0.8%
        72.5%
        42%
        42.5
        SRR27782319
        83.2
        0.00%
        84.7%
        0.27%
        10.2
        73.1
        100.0%
        100.0%
        73.1
        93.9%
        35.3
        SRR27782319_1
        0.8%
        74.7%
        40%
        37.6
        SRR27782319_2
        0.8%
        74.8%
        41%
        37.6
        SRR27782320
        85.2
        0.00%
        84.9%
        0.28%
        10.3
        74.9
        100.0%
        100.0%
        74.9
        93.8%
        36.2
        SRR27782320_1
        0.9%
        72.4%
        40%
        38.6
        SRR27782320_2
        0.8%
        72.6%
        41%
        38.6
        SRR27782321
        90.2
        0.00%
        85.0%
        0.30%
        10.9
        79.2
        100.0%
        100.0%
        79.2
        93.8%
        38.3
        SRR27782321_1
        0.8%
        74.8%
        40%
        40.9
        SRR27782321_2
        0.8%
        73.8%
        41%
        40.9
        SRR27782322
        85.9
        0.00%
        88.1%
        0.30%
        7.7
        78.2
        100.0%
        100.0%
        78.2
        93.7%
        37.9
        SRR27782322_1
        0.9%
        76.3%
        40%
        40.4
        SRR27782322_2
        0.9%
        75.7%
        41%
        40.4
        SRR27782323
        70.5
        0.00%
        89.5%
        0.28%
        5.6
        64.9
        100.0%
        100.0%
        64.9
        94.7%
        31.5
        SRR27782323_1
        0.8%
        73.4%
        41%
        33.3
        SRR27782323_2
        0.9%
        74.3%
        42%
        33.3
        SRR27782324
        91.9
        0.00%
        88.1%
        0.31%
        8.6
        83.3
        100.0%
        100.0%
        83.3
        94.6%
        40.5
        SRR27782324_1
        0.6%
        72.3%
        40%
        42.8
        SRR27782324_2
        0.7%
        72.7%
        41%
        42.8
        SRR27782325
        62.5
        0.00%
        85.2%
        0.28%
        7.3
        55.1
        100.0%
        100.0%
        55.1
        94.0%
        26.6
        SRR27782325_1
        0.8%
        69.4%
        41%
        28.3
        SRR27782325_2
        0.9%
        70.9%
        42%
        28.3
        SRR27782326
        59.5
        0.00%
        88.6%
        0.26%
        5.2
        54.2
        100.0%
        100.0%
        54.2
        95.0%
        26.3
        SRR27782326_1
        0.9%
        72.1%
        41%
        27.7
        SRR27782326_2
        1.0%
        73.5%
        42%
        27.7
        SRR27782327
        115.9
        0.00%
        79.1%
        0.22%
        19.8
        96.1
        100.0%
        100.0%
        96.1
        93.3%
        45.8
        SRR27782327_1
        1.2%
        79.3%
        42%
        49.1
        SRR27782327_2
        1.2%
        80.9%
        43%
        49.1
        SRR27782328
        119.1
        0.00%
        82.5%
        0.23%
        16.9
        102.2
        100.0%
        100.0%
        102.2
        94.2%
        49.1
        SRR27782328_1
        0.9%
        80.3%
        42%
        52.2
        SRR27782328_2
        0.9%
        81.6%
        42%
        52.2
        SRR27782329
        51.0
        0.00%
        78.2%
        0.25%
        9.1
        41.9
        100.0%
        100.0%
        41.9
        92.2%
        20.0
        SRR27782329_1
        0.9%
        71.6%
        42%
        21.6
        SRR27782329_2
        0.8%
        72.9%
        43%
        21.6
        SRR27782330
        74.2
        0.00%
        86.6%
        0.28%
        7.9
        66.3
        100.0%
        100.0%
        66.3
        90.9%
        32.1
        SRR27782330_1
        0.8%
        67.6%
        41%
        35.4
        SRR27782330_2
        0.8%
        67.6%
        42%
        35.4
        SRR27782331
        103.4
        0.00%
        79.1%
        0.38%
        17.8
        85.6
        100.0%
        100.0%
        85.6
        91.9%
        40.9
        SRR27782331_1
        0.7%
        76.4%
        40%
        44.5
        SRR27782331_2
        0.8%
        72.4%
        41%
        44.5
        SRR27782332
        88.4
        0.00%
        87.9%
        0.31%
        8.4
        80.1
        100.0%
        100.0%
        80.1
        93.6%
        38.9
        SRR27782332_1
        0.9%
        74.8%
        40%
        41.5
        SRR27782332_2
        0.9%
        73.3%
        41%
        41.5
        SRR27782333
        93.9
        0.00%
        83.9%
        0.31%
        12.2
        81.6
        100.0%
        100.0%
        81.6
        93.7%
        39.4
        SRR27782333_1
        0.8%
        71.8%
        39%
        42.1
        SRR27782333_2
        0.9%
        72.2%
        40%
        42.1
        SRR27782334
        89.8
        0.00%
        84.2%
        0.30%
        11.4
        78.3
        100.0%
        100.0%
        78.3
        94.1%
        37.8
        SRR27782334_1
        0.9%
        72.1%
        40%
        40.2
        SRR27782334_2
        0.9%
        72.0%
        41%
        40.2
        SRR27782335
        79.4
        0.00%
        83.8%
        0.28%
        10.5
        69.0
        100.0%
        100.0%
        69.0
        93.8%
        33.3
        SRR27782335_1
        0.7%
        72.8%
        41%
        35.5
        SRR27782335_2
        0.7%
        72.6%
        41%
        35.5
        SRR27782336
        134.0
        0.00%
        49.1%
        0.28%
        58.9
        75.1
        100.0%
        100.0%
        75.1
        85.1%
        32.9
        SRR27782336_1
        0.8%
        76.4%
        41%
        38.7
        SRR27782336_2
        0.8%
        76.5%
        42%
        38.7
        SRR27782337
        86.7
        0.00%
        76.2%
        0.27%
        17.3
        69.5
        100.0%
        100.0%
        69.5
        92.0%
        33.0
        SRR27782337_1
        0.9%
        75.9%
        40%
        35.9
        SRR27782337_2
        0.8%
        75.6%
        41%
        35.9
        SRR27782338
        90.1
        0.00%
        72.2%
        0.26%
        20.9
        69.2
        100.0%
        100.0%
        69.2
        90.6%
        32.6
        SRR27782338_1
        1.3%
        75.0%
        38%
        35.9
        SRR27782338_2
        1.1%
        75.6%
        40%
        35.9
        SRR27782339
        81.8
        0.00%
        88.5%
        0.32%
        7.3
        74.5
        100.0%
        100.0%
        74.5
        94.8%
        36.2
        SRR27782339_1
        0.8%
        69.7%
        40%
        38.2
        SRR27782339_2
        0.9%
        69.7%
        41%
        38.2
        SRR27782340
        91.6
        0.00%
        79.8%
        0.32%
        15.3
        76.4
        100.0%
        100.0%
        76.4
        93.2%
        36.6
        SRR27782340_1
        0.8%
        72.2%
        41%
        39.3
        SRR27782340_2
        0.8%
        72.0%
        41%
        39.3
        SRR27782341
        162.0
        0.00%
        51.2%
        0.34%
        68.2
        93.8
        100.0%
        100.0%
        93.8
        85.3%
        41.5
        SRR27782341_1
        0.9%
        76.0%
        41%
        48.7
        SRR27782341_2
        1.0%
        74.9%
        42%
        48.7
        SRR27782342
        89.9
        0.00%
        83.2%
        0.29%
        12.3
        77.7
        100.0%
        100.0%
        77.7
        94.2%
        37.5
        SRR27782342_1
        0.9%
        72.1%
        40%
        39.8
        SRR27782342_2
        1.0%
        72.8%
        41%
        39.8
        SRR27782343
        85.4
        0.00%
        87.5%
        0.32%
        8.4
        77.0
        100.0%
        100.0%
        77.0
        94.4%
        37.4
        SRR27782343_1
        0.8%
        73.4%
        40%
        39.6
        SRR27782343_2
        0.8%
        72.9%
        41%
        39.6
        SRR27782344
        107.5
        0.00%
        53.1%
        0.31%
        43.6
        64.0
        100.0%
        100.0%
        64.0
        86.1%
        28.6
        SRR27782344_1
        0.9%
        73.8%
        38%
        33.1
        SRR27782344_2
        0.9%
        74.2%
        40%
        33.1
        SRR27782345
        92.3
        0.00%
        88.3%
        0.27%
        8.4
        83.9
        100.0%
        100.0%
        83.9
        94.7%
        40.8
        SRR27782345_1
        0.7%
        78.0%
        40%
        43.0
        SRR27782345_2
        0.8%
        78.6%
        41%
        43.0

        STAR_SALMON DESeq2 PCA plot

        PCA plot between samples in the experiment. These values are calculated using DESeq2 in the deseq2_qc.r script.

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        STAR_SALMON DESeq2 sample similarity

        is generated from clustering by Euclidean distances between DESeq2 rlog values for each sample in the deseq2_qc.r script.

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        DupRadar

        provides duplication rate quality control for RNA-Seq datasets. Highly expressed genes can be expected to have a lot of duplicate reads, but high numbers of duplicates at low read counts can indicate low library complexity with technical duplication. This plot shows the general linear models - a summary of the gene duplication distributions.

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        Preseq

        Preseq estimates the complexity of a library, showing how many additional unique reads are sequenced for increasing total read count. A shallow curve indicates complexity saturation. The dashed line shows a perfectly complex library where total reads = unique reads.

        Complexity curve

        Note that the x axis is trimmed at the point where all the datasets show 80% of their maximum y-value, to avoid ridiculous scales.

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        RSeQC

        RSeQC package provides a number of useful modules that can comprehensively evaluate high throughput RNA-seq data.

        Read Distribution

        Read Distribution calculates how mapped reads are distributed over genome features.

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        Inner Distance

        Inner Distance calculates the inner distance (or insert size) between two paired RNA reads. Note that this can be negative if fragments overlap.

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        Read Duplication

        read_duplication.py calculates how many alignment positions have a certain number of exact duplicates. Note - plot truncated at 500 occurrences and binned.

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        Junction Annotation

        Junction annotation compares detected splice junctions to a reference gene model. An RNA read can be spliced 2 or more times, each time is called a splicing event.

           
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        Junction Saturation

        Junction Saturation counts the number of known splicing junctions that are observed in each dataset. If sequencing depth is sufficient, all (annotated) splice junctions should be rediscovered, resulting in a curve that reaches a plateau. Missing low abundance splice junctions can affect downstream analysis.

        Click a line to see the data side by side (as in the original RSeQC plot).

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        Infer experiment

        Infer experiment counts the percentage of reads and read pairs that match the strandedness of overlapping transcripts. It can be used to infer whether RNA-seq library preps are stranded (sense or antisense).

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        Bam Stat

        All numbers reported in millions.

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        Samtools

        Samtools is a suite of programs for interacting with high-throughput sequencing data.

        Percent Mapped

        Alignment metrics from samtools stats; mapped vs. unmapped reads.

        For a set of samples that have come from the same multiplexed library, similar numbers of reads for each sample are expected. Large differences in numbers might indicate issues during the library preparation process. Whilst large differences in read numbers may be controlled for in downstream processings (e.g. read count normalisation), you may wish to consider whether the read depths achieved have fallen below recommended levels depending on the applications.

        Low alignment rates could indicate contamination of samples (e.g. adapter sequences), low sequencing quality or other artefacts. These can be further investigated in the sequence level QC (e.g. from FastQC).

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        Alignment metrics

        This module parses the output from samtools stats. All numbers in millions.

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        Samtools Flagstat

        This module parses the output from samtools flagstat. All numbers in millions.

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        Mapped reads per contig

        The samtools idxstats tool counts the number of mapped reads per chromosome / contig. Chromosomes with < 0.1% of the total aligned reads are omitted from this plot.

           
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        STAR

        STAR is an ultrafast universal RNA-seq aligner.

        Alignment Scores

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        Cutadapt

        Cutadapt is a tool to find and remove adapter sequences, primers, poly-Atails and other types of unwanted sequence from your high-throughput sequencing reads.

        Filtered Reads

        This plot shows the number of reads (SE) / pairs (PE) removed by Cutadapt.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Trimmed Sequence Lengths

        This plot shows the number of reads with certain lengths of adapter trimmed.

        Obs/Exp shows the raw counts divided by the number expected due to sequencing errors. A defined peak may be related to adapter length.

        See the cutadapt documentation for more information on how these numbers are generated.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).